A novel technique for measuring tiny, rapid-fire secretions in the brains and mouthparts of fruit flies (drosophila) is providing insights into the beneficial effects of eating less — information that ultimately could help people suffering from neuromuscular disorders.

Using the method, researchers uncovered never-before-seen brain chemistry that helps explain why fruit flies genetically manipulated to mimic conditions such as Parkinson’s disease and myasthenia gravis are more vigorous and live longer when fed a restricted diet.

The research was conducted by a team from the School of Medicine and the Barshop Institute for Longevity and Aging Studies at The University of Texas Health Science Center San Antonio.

Why eating less may be therapeutic

Senior author Benjamin Eaton, Ph.D., assistant professor of physiology, says the results demonstrate how limiting calories may be therapeutic for people with various syndromes.

Lead author Joel Rawson, Ph.D., and the Eaton team developed a novel system to analyze the impact of diet on life span and motor behavior as well as on neurotransmission, which is believed to underlie most neurological disorders in humans.

Flies on the low-calorie diet showed a 100 percent increase in the release of neurotransmitters. These chemicals carry signals from one nerve cell to another across gaps called synapses. The brain has millions of synapses that are believed to be the critical structures required for normal brain function. Diseases such as Parkinson’s harm them irreparably.

Firing up the muscle activity

The chemicals were secreted at critical locations. “Diet restriction increased the neurotransmitters released at synapses called neuromuscular junctions,” Dr. Eaton said. “These synapses, which form on muscle, transmit nerve impulses from the brain to muscles, resulting in movement. If neuromuscular junctions degenerate, resulting in the release of less neurotransmitter, then muscle activity diminishes. This is observed in diseases such as myasthenia gravis and amyotrophic lateral sclerosis (ALS).”

The team genetically engineered a single pair of motor neurons to develop neurodegenerative disease, resulting in a decrease of the flies’ ability to extend the proboscis, which they use to gather food. “We showed that diet restriction can rescue proboscis extension by increasing the amount of neurotransmitter released. This suggests that diet could be an important therapy for improving muscle function during motor diseases such as ALS.”

Next up is to define the proteins in neurons that are being altered by diet restriction, he said.

An Ellison Medical Foundation New Scholar Award (AG-NS-0415-07) to Dr. Eaton supported this work. Dr. Rawson is supported by grant T32-AG021890 from the National Institute on Aging, National Institutes of Health.